Health Information

Bernstein test

Bernstein test


Acid perfusion test

The Bernstein test is a method to reproduce symptoms of heartburn. It is most often done with other tests to measure esophageal function.

I Would Like to Learn About:

  • How the Test is Performed

    The test is done in a gastroenterology laboratory. A nasogastric (NG) tube is passed through one side of your nose and into your esophagus. Mild hydrochloric acid will be sent down the tube, followed by salt water (saline) solution. This process may be repeated several times.

    You will be asked to tell the health care team about any pain or discomfort you have during the test.

  • How to Prepare for the Test

    You should not eat or drink anything for 8 hours before the test.

  • You may have a gagging feeling and some discomfort when the tube is put in place. The acid may cause symptoms of heartburn. Your throat may be sore after the test.

  • Why the Test is Performed

    The test tries to reproduce symptoms of gastroesophageal reflux (stomach acids coming back up into the esophagus). It is done to see if you have the condition.

  • Normal Results

    The test results will be negative.

  • What Abnormal Results Mean

    A positive test hows that your symptoms are caused by esophageal reflux of acid from the stomach.

  • Risks

    There is a risk of gagging or vomiting.

Related Information

  HeartburnEsophageal manomet...Gastroesophageal r...     Gastroesophageal r...


Fass R, Achem SR Noncardiac chest pain: diagnostic evaluation. Dis Esophagus. 2012 Feb;25(2):89-101. PMID: 21777340 www.ncbi.nlm.nih.gov/pubmed/21777340.

Kahrilas PJ, Pandolfino JE. Esophageal neuromuscular function and motility disorders. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease. 9th ed. Philadelphia, PA: Elsevier Saunders; 2010:chap 42.


Review Date: 11/20/2014  

Reviewed By: Jenifer K. Lehrer, MD, Department of Gastroenterology, Frankford-Torresdale Hospital, Aria Health System, Philadelphia, PA. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.

The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.


A.D.A.M. content is best viewed in IE9 or above, Firefox and Google Chrome browser.